MIRO

Bruker's Microscope Image Registration and Overlay (MIRO) software integrates AFM imaging with optical microscopy. This optional software allows the user to automatically import and rescale light microscope images, which can then be used to direct the location of AFM imaging and force measurements. Correlated datasets can be captured and analyzed with the powerful offline functionality.

Figure 1: Fixed macrophage cell labeled with Alexa Fluor phalloidin (red) and DAPI (blue) with AFM inset. Image courtesey of Peter Hanley, Institute fro Physiology II, University of Muenster, Germany.

If you would like more information or would like to purchase MIRO, please contact Bruker.

MIROTM (Microscope Image Registration and Overlay) user-guided interactive software allows you to do the following:

• Register optical and AFM images
• Import and overlay multiple optical and AFM images
• Select regions of interest on registered optical images for subsequent acquisition of AFM images or data in real time
• Adjust and fine-tune the registration parameters of both optical and AFM images
• Store and restore profiles for different system configurations (e.g. multiple objective lenses)
• Adjust image color, transparency and overlay options—individually for each channel
• Export overlaid images
• Save each overlay and associated image parameters for further analysis in offline mode
• MIRO 2.0 adds multiple Region of Interest views which allow you to more easily compare overlaid images

Open topic with navigation