Two micro manipulators manufactured by Kleindiek are installed inside the FEI Nova 200 on a special extra base. Here is a brief user manual how to use these manipulators. Please note! Reading this web-page is not enough to be able to use these manipulators in a safe way. You MUST receive training from a person responsible for these!
	Here are the two manipulators mounted on the special extra base-plate installed inside the FEI Nova 200.
	Each manipulator has three parts for each movment direction: A: Left / Right movement B: Up / Down movement C: In / Out movement The "Quickfoot" part is clamped to the special base plate with a screw and can be adjusted by the tool responsible person.
	This is the controller for one of the manipulators, the three first knobs control the respective motion direction.
	Here is a more convenient controller (which many of you may recognise). Both manipulators can be controlled with this. The two joy sticks control the motions as indicated.
	Here is the On / Off button.
	The speed of the motion is controlled by these two buttons. Please note that the highest speed (6) can only be used when the stage is open for mounting samples etc. When the stage is inside the chamber, the risk of pushing a tip into a column, a wall, your sample, etc. is 100%.
	You can switch between the two manipulators with the right front button.
The driving principle is using a piezoelectric motor, a "NanoMotor" There are two modes this motor can operate in: Fine mode: 1 µm movment divided in 4095 steps in / out motion 20 µm divided in 4096 steps left / right / up / down. This mode is used in speed ranges 1 and 2. Coarse mode: Ratchet motion, used in speed ranges 3 - 6. Question for Andreas: how big is on step in coarse mode?
	In order to take one coarse step (length of step depends on speed setting) you press one of the indicated buttons. Up / Down, Left / Right, In / Out ????
Considerations Absolute positions are not available. There is no way to "go to home position" for any axis of the manipulator. Exact size of step difficult to calculate. Each nanocontrol for one manipulator, voltage needed to maintain position ?????? Maximum range in fine mode (1 µm in/out, 20 µm left/right, up/down.
	Speed & control setting The speed and which manipulator is active with the controller is indicated on the display (which display??????) As we have only two manipulators installed we only have left and right. Use highest speed 6 ONLY with open load lock!
	Vibration can be applied to the tip of the manipulator, this is very useful to decide when tip makes contact with sample surface. Visual aid for fast and safe approach Vibration amplitude decreases when in contact Operate from controller box
	Handling Avoid applying pressure onto forks of the horizontal and vertical axes. Pressure higher than 2 N will damage the high preceision bearings.
	Handling Only handle at back grip Insert & remove tips for each use??????? Leave manipulators inside FIB Park in safe positions
	Handling Do not get in touch with the delicate wires.
	Handling tips
	Preparing a new tip ‐ staff only Please note that the actual tip (not visible in the picture) is welded to the side of the wire.
	Mounting tip Hold by white ring! When inserting or removing a tip holder, point the vertical axis of the manipulator directly upward and hold the manipulator firmly by the white ring at the front of the linear axis. If access from above is restricted then either move the axis as far upward as it will go or point it directly downward. Use pliers to insert or remove the tip holder. Insertion only a few mm!
	Rotator Length: 32 mm Diameter: 4 mm Weight: 2 g Travel: 360° unlimited Resolution: 0.1° Torque: 0.01 mNm Temperature range: 273 K to 353 K Lowest pressure: 10-7 mbar
	System view from above Rotator is mounted to the right.
	Manipulators are mounted on the base with friction fasteners — some adjustment possible.
	Connectors and wires Be careful with wires when loading and unloading. Check there is enough range for tilts.
	Needles / tips Select tip depending on purpose Do not destroy sharp tips (scratching, glue etc.) FIB your own tip Keep within known position: retract and vent+reload if blind. Rotation: tip at rotation axis, i.e. never mount an angled tip on the rotator
	Position tips within 2 mm of center Hard to see actual tips May crash on: Stub Sample Each other Column
	CCD camera view View from inside of chamber Watch when: Loading Rotating Adjusting Z pos Do not squeeze manipulators into column!
	Locate needles after loading Should be within 3 mm of stage center If not visible: Vent, reposition and reload
	Check needle rotation orientation Rotator needle may touch only when on the bottom side of the wire.
	Rmember tip holder?????? Rotation tricky ???????
	Tip shape This is how fresh sharp tips should look like
	Sharp tips — FIB:ed
Scratching deposited layers
Scratching... Effect depends on speed and angle
Moving small objects
	SEM-glue Glue cured by the electron beam Manipulate: Less than 100 pA Cure: 1 nA, 50,000 magn. or zoom-window Wait 1 minute Bonding strength 2 mN Fully cure: High beam currents Large voltages
Demo movies    http://www.nanotechnik.com/nanomanipulation.html http://www.nanotechnik.com/mm3a-em.html