Start icon on desktop looks like
this:
| This is a one-window software, you immediately see all functions in one window. Top left are drop down menues and function buttons. Top right are another set of function buttons, hopefully self-explanatory. To the right are control functions for selecting magnification to get a calibrated scale bar, annotation, intensity profile. Also exposure settings, quality (image size). Below this are buttons to start /stop live camera capture. |
| Here are the drop down menues and function buttons at the top left of the window. They should be fairly self explanatory. "Point" is for moving annotation objects, moving the scale bar. "AE Area" is for selecting which area in the live picture should be used for automatic exposure. |
|
At the top right are function buttons for the image size displayed on
screen.
Below are function buttons to turn on scale bar and select which magnification is used. Next is annotation, lines, squares, circles, text. Last is button to turn on/off an intensity profile (only horizontal). Next are resolution selectors for live image ("Fast (Focus)") and capture. Exposure control, either automatic or manual. Exposure time and Gain can be adjusted in manual mode. The "Color" part gives you control over contrast and white balance. Advanced ... At the bottom are buttons to start and stop the live image capture and capturing sequences of pictures. |
|
At the bottom of the window is a minidisplay of the pictures currently
captured in the default picture folder,
currently D:\MicroscopePictures. Please do not change
this, it is on a separate 1 TB disk, so there is plenty of space to
store pictures.
Please create your own folder here for your pictures. In this screen shot this folder is empty, it was newly created. At the very bottom is an indicator of the frame rate during live imaging, here showin 14.70 Fps (Frames per second). |
|
There is also an indicator of ......., the size of a pixel if
calibration has been done earlier (it has). Next is [X,Y] RGB: (XXX,
XXX, XXX) which are coordinates in the picture and the corresponding
RGB values for that coordinate.
Last there is an indicator of picture mode and size in pixels. |
| Here are shown the selection of modes for live imaging. The fastest is the 640x476 Central. This is cutting out the central pixels in the image and displaying this for a good way to set the focus as good as possible. Here fram rate is usually 14 fps. The other modes give about 5-6 fps. |
| The possible magnification settings to select from. Please note that ONLY the 5X, 10X, 20X, 50X and 100X have been calibrated, these are the objectives available on this microscope. |
| Here are the alternatives for picture capture shown. You probably want to use the "Fine" setting, giving you the full 5 MPixels of the camera. |
| Here is the function button for "Profile" active, giving the result shown below. By dragging on the dotted line you can move the profile vertically in the live picture. |
