Electron beam lithography (EBL) is of major importance for
ultraminiaturized biohybrid system fabrication, as it allows combining
biomolecular patterning and mechanical structure definition on the
nanoscale. Existing methods are limited by multistep biomolecule
immobilization procedures, harsh processing conditions that are
harmful to sensitive biomolecules, or the structural properties of the
resulting protein monolayers or hydrogel-based resists. This work
introduces a thiol-ene EBL resist with chemically reactive thiol
groups on its native surface that allow the direct and selective
"click" immobilization of biomolecules under benign processing
conditions. We constructed EBL structured features of size down to 20
nm, and direct functionalized the nanostructures with a sandwich of
biotin and streptavidin. The facile combination of polymer
nanostructuring with biomolecule immobilization enables mechanically
robust biohybrid components of interest for nanoscale biomedical,
electronic, photonic, and robotic applications.
See more at https://pubs.acs.org/doi/10.1021/acsnano.8b03709 Here also PDF-version |