Usage

Place the Sample

1. Arrange the clamps approximately as shown in Figure 1 to minimize the danger of crashing the objective or the head into the clamps.

Figure 1: Sample clamp arrangement

2. To use the clamps on samples thicker than 1 mm, place 1/8" OD by 1/16" thick spacer magnets (supplied) under the magnets integrated into the clamps as shown in Figure 2.

Figure 2: Sample clamp with two spacer magnets installed below.

3. Moving the clamps is most easily accomplished by pushing down on the rear of the clamp as shown in Figure 3.

Figure 3: Push down at the rear of the clamp to release it.

4. Route the probe cable to the desired port on the terminal block.
5. Attach a Kapton tape disk (supplied) to the bottom of the 1/2" diameter magnet to avoid scratching the chuck.
6. Place the magnet to hold the wire lead from the probe onto the chuck as shown in Figure 4.

Figure 4: Hold the cable to the chuck with a 1/2" magnet

Set Up the Sample Travel

The maximum Y axis travel is 30 mm, shown in Figure 5.

Figure 5: Maximum Y axis travel

Bruker suggests that you constrain the clamps so that they intrude no more than 2 mm on each side of the sample. See Figure 6. Placing the clamps further inboard of the sample edges will reduce the available travel.

Figure 6: Suggested clamp constraints

The area that is unavailable after placing the clamps 2 mm inboard is 4.25 mm in the Y axis and is illustrated in Figure 7.

Figure 7: Unavailable area with clamp locations as described in Figure 6.

Focus the Backside Illumination Optics Assembly on the Sample

	The focus can be adjusted from approximately the top of the chuck surface to about 3 mm above it. Ensure that the head is above the sample clamp height and move the chuck so that the sample is under the probe tip.
	Click the Navigate icon in the Workflow toolbar.
	Click the Find Surface icon and record the Z height, displayed in the NanoScope status bar, shown in Figure 8.
	If not using TappingMode or PeakForce TUNA, manually focus on the surface and record the Z height.

Figure 8: Z height display in the NanoScope status bar

Check that the microscope illumination is low enough to see the optical fiber, shown in Figure 9. NOTE: The camera image may differ depending on the sample opacity.

Figure 9: Optical fiber visible at low illumination

Adjust focus (Z stage) of the Backside Illumination Optics Assembly so that the optical fiber is back in focus.

Adjust the X-Y position of the light source

Because of the limited spot size (200 μm diameter), the light spot must be centered in the scan range especially for experiments using large sample scan sizes.

The light spot position must be readjusted after a probe change.

Figure 10: The Backside Illumination Optics Assembly stages Clockwise rotation of the Z translation knob moves the Z stage down while counterclockwise rotation moves it up.

Troubleshooting

If you see a large X-Y movement while adjusting Z, check both the vacuum holding the Backside Illumination Optics Assembly to the granite base (by pulling on the handles) and the vacuum holding the X-Y stage (by pulling on the Y translate screw).

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