Nikon DIC ME600 microscope with Nikon 5 Mpix camera

Responsible: Anders Liljeborg

Microscope similar to the old one that has been in the NanoFabLab for several years. However this has better objectives and a much better digital 5 Mpix camera.

It is a Nikon model ME600 epi-illumination microscope with DIC (Differential Interference Contrast) which gives the possibility to see height differences in reflecting specimens displayed as color differences in the image.

It also have a Dark Field option, which means that the illumination is indirect, producing images with dark background and edges and features displyed brightly.

It has objectives from the CFi Plan series, newer version with much longer working distance than the old microscope.

Magnification Numerical Aperture
Working Distance
0.15 18 mm
10× 0.30 15 mm
20× 0.45 4.5 mm
50× 0.80 1 mm
100× 0.90 1 mm

The camera is Nikon camera head DS-5M with camera control unit DS-U1

Description of microscope controls, same as "old" microscope,
except Neutral Density (ND) filter not present.

NIS 5 Documentation software for picture acquisition

Older version NIS 4.0.0 software for picture acquisition, kept for reference

Specifications, camera DS-5M and control unit DS-U1

CCD: 2/3 in. high density CCD; total number of pixels: 5.24 million (effective 5.07 million)
Sensitivity: 2400 lx, F5.6 or greater; equivalent to ISO 260
A/D conversion: 12-bit
Lens mount: C-mount
Exposure time: 1/1000 to 60 sec
Exposure control: Program AE, Shutter-priority, Focus AE, manual, with AE lock function
Exposure correction: Correction range +2.0EV, Step: 1/3EV
Digital zoom: 5 to 2400%
Interval shooting: 5 sec. - 12 hr. intervals
Exposure metering: Average metering, Peak hold metering
White balance: Set method, Color balance adjustable
Compensation: Brightness, Contrast, Gamma correction, Rotation, Flip-flop, Crop, Shading correction; Monochrome, Negative
Storable image size: 2560 x 1920 pixels, 1280 x 960 pixels, 640 x 480 pixels
Storage format: BMP, TIFF, JPEG, JPEG2000
Live display mode: Center scan mode (15 frames/sec. max), 1.3M interlace mode (6.8 frames/sec. max. 2x2 binning mode); 1.3M progressive mode (7.5 frames/sec. max), 5M interlace mode (3.75 frames/sec. max)
Interface: USB 2.0 Device Port (computer control connector), USB1.1 Host Port (microscope connection)
Power supply: AC100-240V 50/60Hz
Power consumption: 43VA
Dimensions: DS-5M Camera head: 90.6 (W) x 40.9 (H) x 75.3 (D) mm; DS-U1 Control Unit: 180.5 (W) x 68.4 (H) x 144.5 (D) mm
Weight: DS-5M Camera head: approx. 230g; DS-U1 Control Unit: approx. 1000g
Operating environment: 0-40 degrees C, 85% RH max. (without condensation)
System composition: DS-5M Camera head, Camera Cable (3m), DS-U1 camera control unit, power cord
Optional accessories: 0.7x Relay Lens (C-mount), support kits
Operating systems: Windows 2000/XP (English & Japanese versions)
Software: Nikon ACT-2U software, EclipseNet software, or DS-U1 TWAIN-Compliant Software.
Anders Liljeborg Nanostructure Physics, KTH.